bsrG [2019-07-03 11:12:57]

hydrophobic toxin; interferes with cell envelope biosynthesis, causes membrane invaginations together with delocalization of the cell wall synthesis machinery, triggers autolysis, and inhibits protein biosynthesis

locus: new_2273515_2273748

pI: 0.00

mw: 0.00 kDa

gene length: 234 bp

function: stability of prophage SPß

product: hydrophobic class I toxin

essential: no

synonyms: bsrG,S809

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.17|Toxins, antitoxins and immunity against toxins] → [category|SW 4.3.17.1|Type 1 TA systems]

Gene

Coordinates: 2,273,515 → 2,273,748

The protein

[SW|Localization]

• membrane protein (based on the presence of a trans-membrane domain) [Pubmed|22229825]

Expression and Regulation

Operons

• genes: [gene|24A1B22DE468F187B6631D3D4E214B64CB8BB4DD|bsrG]

  description

  • [pubmed|22229825]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22229825], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • expression increases at the onset of the stationary phase [pubmed|22229825]
  • the mRNA is destabilized upon interaction with the antitoxin RNA [gene|10A53E2465B5EAA307A099052FBBDF3B62857291|SR4] [pubmed|22229825]

  regulatory mechanism

  • the mRNA is degraded by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III], [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] and [protein|0472A4346ADA1DC41C9D00E8F3222A0EBB76DFA8|RNase R], degradation is enhanced at higher temperature [pubmed|22229825]

  Additional information

  • half-life of the mRNA: 15 min [pubmed|22229825]
  • upon heat shock, the mRNA refolds and is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] and [protein|3EB289D0F58A58C693AB588798EE66A731341999|RNase J1][pubmed|22229825]

labs

• [SW|Sabine Brantl], Jena, Germany

References

Reviews

• 31075979

Original publications

• 18948176,20525796,23969414,22229825,26234942,26802042